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mgmt promoter methylation detection kit  (EntroGen Inc)

 
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    Structured Review

    EntroGen Inc mgmt promoter methylation detection kit
    (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of <t>MGMT</t> promoter <t>methylation</t> (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001
    Mgmt Promoter Methylation Detection Kit, supplied by EntroGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgmt promoter methylation detection kit/product/EntroGen Inc
    Average 90 stars, based on 1 article reviews
    mgmt promoter methylation detection kit - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Mitochondrial sites of contact with the nucleus aid in chemotherapy evasion of glioblastoma cells"

    Article Title: Mitochondrial sites of contact with the nucleus aid in chemotherapy evasion of glioblastoma cells

    Journal: bioRxiv

    doi: 10.1101/2024.08.27.608373

    (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of MGMT promoter methylation (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001
    Figure Legend Snippet: (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of MGMT promoter methylation (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001

    Techniques Used: Staining, Methylation, Western Blot



    Similar Products

    mgmt promoter methylation detection kit  (EntroGen Inc)
    90
    EntroGen Inc mgmt promoter methylation detection kit
    (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of <t>MGMT</t> promoter <t>methylation</t> (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001
    Mgmt Promoter Methylation Detection Kit, supplied by EntroGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgmt promoter methylation detection kit/product/EntroGen Inc
    Average 90 stars, based on 1 article reviews
    mgmt promoter methylation detection kit - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of MGMT promoter methylation (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001

    Journal: bioRxiv

    Article Title: Mitochondrial sites of contact with the nucleus aid in chemotherapy evasion of glioblastoma cells

    doi: 10.1101/2024.08.27.608373

    Figure Lengend Snippet: (A) Cell viability was evaluated via crystal violet in U87MG cells treated with Etifoxine (30μM) and in combination with TMZ (100μM) for 48 and 72 hours. (B) Representative images of U87MG spheroids stained with Dapi, PI and antibody for TSPO at resting conditions, following TMZ alone and combined with Etifoxine (TMZ+Etifoxine). Histograms reporting quantification of spheroids diameter in the conditions of analysis ( C ) and relative changes in fluorescent intensity ( D ). (E) Percentage of MGMT promoter methylation (MGMTp meth%) in t98G and ADF cell lines. (F) Immunoblotting of MGMT and TSPO in ADF total lysates treated with TMZ (100μM) alone or combined with Lovastatin (10μM) and Etifoxine (30μM) for 24 hours. The graph in panel ( G ) shows the relative densitometry of MGMT and TSPO normalized to GAPDH. All data are represented as mean±sem. *p≤0.05; **p≤0.01; ***p≤0.001

    Article Snippet: We used the MGMT Promoter Methylation Detection Kit (EntroGen, CA, USA) following the manufacturer’s instructions.

    Techniques: Staining, Methylation, Western Blot